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easysep human apc-positive selection magnetic bead separation  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc easysep human apc-positive selection magnetic bead separation
    Easysep Human Apc Positive Selection Magnetic Bead Separation, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+apc-positive+selection+magnetic+bead+separation/easysep+human+apc+positive+selection+magnetic+bead+separation/pmc03685392-48-17-24
    Average 90 stars, based on 1 article reviews
    easysep human apc-positive selection magnetic bead separation - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Progenitor Cells Identified by PDGFR-Alpha Expression in the Developing and Diseased Human Heart
    Article Snippet: Briefly, mononuclear cells were isolated from the dissected hearts by mincing the tissue before digestion in Liberase Blendzyme (Roche; 1:50) in PBS at 37°C for 30 min with triturating mechanically at 10-min intervals and removing the myocyte debris with a 40-μm filter. .. The resulting cells were then subjected to incubation with APC-conjugated mouse monoclonal anti-PDGFRα antibody (R&D; 1:10) and EasySep Human APC-Positive Selection magnetic bead separation (Stem Cell Technologies) according to the manufacturer's instructions. .. Isolated cells were plated on a tissue culture plastic and cultured in a basal medium (80% knockout Dulbecco's minimum essential medium [DMEM; Invitrogen], 10% fetal bovine serum [FBS; HyClone], 1 mM l -glutamine [Gibco], 0.1 mM β-mercaptoethanol, 1% nonessential amino acid stock [Gibco], penicillin G 100 U/mL [Cellgro], and streptomycin 100 mg/mL [Cellgro]) at 37°C and 5% CO2.

    Selection:

    Article Title: Progenitor Cells Identified by PDGFR-Alpha Expression in the Developing and Diseased Human Heart
    Article Snippet: Briefly, mononuclear cells were isolated from the dissected hearts by mincing the tissue before digestion in Liberase Blendzyme (Roche; 1:50) in PBS at 37°C for 30 min with triturating mechanically at 10-min intervals and removing the myocyte debris with a 40-μm filter. .. The resulting cells were then subjected to incubation with APC-conjugated mouse monoclonal anti-PDGFRα antibody (R&D; 1:10) and EasySep Human APC-Positive Selection magnetic bead separation (Stem Cell Technologies) according to the manufacturer's instructions. .. Isolated cells were plated on a tissue culture plastic and cultured in a basal medium (80% knockout Dulbecco's minimum essential medium [DMEM; Invitrogen], 10% fetal bovine serum [FBS; HyClone], 1 mM l -glutamine [Gibco], 0.1 mM β-mercaptoethanol, 1% nonessential amino acid stock [Gibco], penicillin G 100 U/mL [Cellgro], and streptomycin 100 mg/mL [Cellgro]) at 37°C and 5% CO2.

    Stem Cell:

    Article Title: Progenitor Cells Identified by PDGFR-Alpha Expression in the Developing and Diseased Human Heart
    Article Snippet: Briefly, mononuclear cells were isolated from the dissected hearts by mincing the tissue before digestion in Liberase Blendzyme (Roche; 1:50) in PBS at 37°C for 30 min with triturating mechanically at 10-min intervals and removing the myocyte debris with a 40-μm filter. .. The resulting cells were then subjected to incubation with APC-conjugated mouse monoclonal anti-PDGFRα antibody (R&D; 1:10) and EasySep Human APC-Positive Selection magnetic bead separation (Stem Cell Technologies) according to the manufacturer's instructions. .. Isolated cells were plated on a tissue culture plastic and cultured in a basal medium (80% knockout Dulbecco's minimum essential medium [DMEM; Invitrogen], 10% fetal bovine serum [FBS; HyClone], 1 mM l -glutamine [Gibco], 0.1 mM β-mercaptoethanol, 1% nonessential amino acid stock [Gibco], penicillin G 100 U/mL [Cellgro], and streptomycin 100 mg/mL [Cellgro]) at 37°C and 5% CO2.



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    90
    STEMCELL Technologies Inc easysep human apc-positive selection magnetic bead separation
    Easysep Human Apc Positive Selection Magnetic Bead Separation, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+apc-positive+selection+magnetic+bead+separation/easysep+human+apc+positive+selection+magnetic+bead+separation/pmc03685392-48-17-24
    Average 90 stars, based on 1 article reviews
    easysep human apc-positive selection magnetic bead separation - by Bioz Stars, 2026-10
    90/100 stars
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